Real-time Polymerase Chain Reaction:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
SYBR Green Assay:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
Quantitation Assay:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
Lysis:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
Extraction:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
Negative Control:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
In Situ Hybridization:Article Title: Identification of Circular RNAs Regulating Cardiomyocyte Proliferation in
Article Snippet: .. 1 Chemicals Source Cat. No B27 supplement Gibco 17504044 B27 supplement minus insulin Gibco A1895601 Bovine Serum Albumin (BSA) Thermo Fisher Scientific BP9706100 CHIR99021 BioGems 2520691 D-Luciferin Sigma-Aldrich L9504 DMEM Corning MT10013CV DPBS Gibco 14190250 Dimethyl sulfoxide Sigma-Aldrich 67-68-5 DAPI Invitrogen D1306 High-Capacity RNA-to-cDNATM Kit Applied BiosystemsTM 4387406 iPS DF19-9-7T WiCells Inc FTDL-01 IWR-1 BioGems 1128234 KAPA SYBR FAST qPCR Kits Roche 07959486001 Illumina Ribo-Zero Plus rRNA Depletion Kit Illumina Inc. 20037135 LightCycler 480 SYBR Green I Master Roche Inc 04887352001 LipofectamineTM RNAiMAX Thermo Fisher Scientific 13778-100 Matrigel Thermo Fisher Scientific CB356253 mTeSRTM Plus kit Stem Cell Technologies 100-0276 MicroRNA first-strand synthesis and miRNA quantitation kit Takara Bio USA 638316 NEBNext® UltraTM II Directional RNA Library Prep Kit New England Biolabs E7760L Newborn Calf Serum, New Zealand origin Thermo Fisher Scientific 16010159 Propidium Iodide - 1.0 mg/mL Solution in Water Thermo Fisher Scientific P3566 QubitTM RNA High Sensitivity (HS) Assay Kits Thermo Fisher Scientific Q32852 RPMI 1640 Gibco 11875119 RPMI 1640 with no glucose Gibco 11879020 RNeasy mini kit QIAGEN 74104 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89901 Silencer® Select Negative Control #1 siRNA Thermo Fisher Scientific 4390843 Sodium azide Sigma Aldrich Inc. 26628-22-8 Two-well Chamber Slide Thermo Fisher Scientific 154852 TNNT2 RNA Alexa Fluor 568 probe Thermo Fisher Scientific PF-210 ViewRNATM ISH Cell Assay Kit Thermo Fisher Scientific QVC0001 1 Supplemental table 7. .. Primary and secondary antibodies 1 Chemicals Dlutions Source Cat. No Cardiac Troponin T 1:100 Abcam ab8295 BD PharmingenTM Alexa Fluor® 647 Mouse Anti-Cardiac Troponin T 1:50 BD Biosciences 565744 BrdU (5-bromo-2'deoxyuridine) 1:100 Abcam ab142567 PH3 1:200 Sigma Aldrich Inc. 06-570 Alexa Fluor® 488 AffiniPure Donkey AntiRat IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 712-545-150 Alexa Fluor® 488 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-545-152 Alexa Fluor® 594 AffiniPure Donkey AntiRabbit IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 711-585-152 Alexa Fluor® 594 AffiniPure Donkey AntiMouse IgG (H+L) 1:100 JACKSON IMMUNORESEARCH 715-585-150 2 Supplemental table 8.
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Article Title: Gephyrin promotes autonomous assembly and synaptic localization of GABAergic postsynaptic components without presynaptic GABA release
Article Snippet: From day 15 onward, N3 media was progressively switched to Neurobasal Plus media (Thermo Fisher) supplemented with B27 (Thermo Fisher) + 5% FBS + 1% penicillin-streptomycin (Thermo Fisher).
Cell Culture:Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
Article Snippet: .. For neurogenic, SSCs were cultured on laminin-coated dishes in neurobasal medium (10888022, Thermo Fisher, USA) supplemented with B27 supplement (17504044, Thermo Fisher, USA), 1 % penicillin, 20 ng/mL epidermal growth factor (E9644, Sigma-Aldrich, USA) and 40 ng/mL fibroblast growth factor (450-33-1 MG, Thermo Fisher, USA). .. After 4 weeks, cells were incubated overnight at 4 °C with primary mouse anti-Nestin antibody (1:100, MA1-110, Thermo Fisher, USA), followed by incubation with Alexa Fluor 647-conjugated secondary antibody Goat anti-mouse IgG (1:1000, ab150115, Abcam, UK) for immunofluorescence staining to assess neurogenic differentiation [ ].
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